Abstracts must be submitted by 7 June 2003.

Papers must be received by 1 August 2003.

If changes are required as a result of discussions at the meeting, these will be made either at the meeting or within one week of the meeting.
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1. Margins are 1.0 inches or 2.54 cm all around. Left justified.

2. Basic tabs are at 1-1/4 and 1-1/2 inch or 3.2 and 3.8 cm. Others may be used for special indentation

3. Font is Times, size 12 point. All text is justified. Table and figure captions are also justified.

4. Titles are all caps, bold.

5. Authors are regular type, one line space, no bold. Names of the authors are given name followed by family name. The place where the work was performed is given in the next 2 or 3 lines. Institute, street address, city, state, country. The address must be sufficient for mailed inquiries.

6. The paper starts one line space after authors.

7. Major headings in paper are bold, all caps (ABSTRACT, INTRODUCTION, METHODS, RESULTS, CONCLUSIONS, REFERENCES, KEY WORDS, FIGURES AND TABLES, etc.) If you have sent the file as a text only file, the headings will not be bold. This is OK. We would rather add the bold style than take it out.

8. One line space before each major heading

9. Put all tables and figures at the end of each paper.

10. No more than 3 tables and 3 figures

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12. Key words should be listed after the references and before the tables and figures.

Rudolf Portmann and Ruth Holzer
NC Laboratory Spiez
CH-3700 Spiez, Switzerland

INTRODUCTION
Toxicity assessment with laboratory animals faces more and more obstacles, therefore one is forced to look for alternatives. On the other hand legislation is still demanding toxicity assay with such animals. Nevertheless there is place for alternative toxicity assays, if they are more cost ...

MATERIALS AND METHODS
Cysts: Artemia franciscana cysts were purchased from the artemia reference center of the university Ghent in Belgium, but cysts from a local aquarium shop originally collected in the Great Salt Lakes (Utah, USA) gave similar results.
Chemicals: The inorganic salts used for the artificial sea water were of analytical grade obtained from Merck (Darmstadt, Germany). Most of the test substances were purchased from Sigma...

RESULTS AND DISCUSSIONS
The brine shrimp, Artemia salina, has a world wide distribution and is in most cases bisexual. The adult female and male animal live in salt lakes and brine ponds found all over the world. Artemia cysts can be stored under oxygen exclusion for years without losing viability, and as soon they are immersed in salt water, the nauplii hatch. Artemia has therefore an almost unique advantage, since it does not require any maintenance or stock culturing.
Toxicity assays have to be done under strictly controlled conditions (1), since biological systems still have enough degrees of freedom for impeding to reach exact data, as it is known in ...

SUMMARY
A semiautomatic screening assay for toxic samples is described. The assay uses ...

REFERENCES

1. Portmann, R. and Holzer R. (1995) Proc. 5th Int. Symp. Protect. Chem. Biol. Warfare Agents, Stockholm, Sweden, 421-428.
2. Sorgeloos, P. and Persoone, G. (1975) Aquaculture 6, 303-317.
3. Michael, A.S. et al. (1956) Science 123, 464.
4. Vanhaecke, P. et al. (1981) Ecotoxicol. Environ. Saf. 5, 382-387.

KEY WORDS
Toxicity, assay, artemia, method, microplate, semi-automatic

FIGURES AND TABLES
Figure 1. Schematic of artemia.

Table 1. A comparison of the results of the Auto-Tox method with other methods for the determination of chemical toxicity.

End of Example.